Canthaxanthin chromatography

A TLC method for analysis of bixin, lycopene, canthaxanthin, and P-apo-8 -carotenal was recently presented (104) for rapid screening of food color adulteration. Another method that was quickly supplanted by HPLC was that of liquid-gel partition chromatography, using stationary phases such as Sephadex LH-20 or hydroxyalkoxypropyl Sephadex (50). This method, which is capable of resolving retinyl esters, retinol, retinal, and retinoic acid, was applied to studying vitamin A metabolism, but it is slower and gives less resolution than HPLC on sturdy silica stationary phases. 

T Van Vliet, F Van Schaik, J Van Schoonhoven, J Schrijver. Determination of several retinoids, carotenoids and E vitamers by high performance liquid chromatography. Application to plasma and tissues of rats fed a diet rich in either [J-caro-tene or canthaxanthin. J Chromatogr 553 179-186, 1991. 

Retina, crystal deposition Methoxyflurane [50] canthaxanthin [51] High-performance liquid chromatography... 

FIGURE 6.14 Carotenoid high-performance liquid chromatography (HPLC) profile of a pigment extract from animal product (egg-yolk). Peaks 1, canthaxanthin 2, lutein 3, zeax-anthin 4, a-carotene 5, (i-carotene. Mobile phase A acetonitrile, B dichloromethane/hexane (1 1), and C methanol flow rate, 0.7 mL/min detection at 470 nm. (From Khachik, F. et al.. Methods Enzymol., 213, 205, 1992.)... 

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