Cannabinoid radioimmunoassay

HPLC cleanup of biological fluids has been reported prior to cannabinoid analysis by direct mass spectrometry, (1) GC-mass spectrometry, (Wall, this vol., Clarks, this vol.), GC (2) and radioimmunoassay (Teale, this vol.). 

Since radioimmunoassay has permitted the quantitation of substances present in concentrations as low as a few pg/ml of a biological fluid, and since the inherent selectivity of antibodies often make sample preparation requirements minimal and assay methodology simple, RIA has naturally been considered as a means for measuring blood levels of cannabinoid compounds. 

Antisera Raised Against Tetrahydrocannabinol in the Radioimmunoassay of Cannabinoids... 

Radioimmunoassay. In body fluids cannabinoid metabolites, sensitivity 10 ng/ml for blood and 2 ng/ml for urine—idem, 14-18. 

Gross SJ, Worthy TE, Nerder L, Zimmermann EG, Soares JR, Lomax P (1985) Detection of recent cannabis use by saliva delta-9-THC radioimmunoassay. J Anal Toxicol 9 1-5 Grotenhermen F (2003) Pharmacokinetics and pharmacodynamics of cannabinoids. Clin... 

Hanson VW, Buonarati MH, Basel RC, Wade NA, Yep C, Biasotti AA, Reeve VC, Wong AS, OrbanowskyMW (1983) Comparison of3H-and 1251-radioimmunoassay and gas chromatography/mass spectrometry for the determination of delta-9-tetrahydrocannabinol and cannabinoids in blood and serum. J Anal Toxicol 7 96-102... 

AJ Clatworthy, et al. Gas chromatographic mass spectrometric confirmation of radioimmunoassay results for cannabinoids in blood and urine. Forensic Sci Int 46 219, 1990. 

The Detection and Quantification of Cannabinoids in Blood and Urine by Radioimmunoassay (RIA), High-Performance Liquid Chromatography (HPLC)/RIA and Gas Chromatography/... 

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