Candidate gene profiling

Bertucci F et al. Gene expression profiling of primary breast carcinomas using arrays of candidate genes. Hum Mol Genet 2000 9 2981-2991. 

The peppermint oil gland secretory cell cDNA library has proven to provide a highly enriched source of candidate genes involved in essential oil biosynthesis. A functional genomics approach has successfully been employed to clone genes involved in the mevalonate-independent pathway of isoprenoid biosynthesis and in the peppermint-specific steps producing (-)-menthol and (-)-menthone. The optimization of LC-MS technology to profile phosphoiylated carbohydrates and... 

Figure 15-4 In silico subtraction method for mechanistic study to compare the hepatic gene expression profile induced by compound X to other compounds that induce hepatic APR secondary to inflammation at sites other than the liver. In the proposed simple model, compound X-induced gene expression change has two components. One is vasculitis specific the other is acute phase response (APR) specific. By subtracting the APR specific component, we might select candidate genes that are specifically associated with the hepatic vascular lesion Diclofenac known to induce APR without triggering vasculitis in liver is used to subtract genes activated by hepatic APR from the compound X response.

Bleeker FE, Lamba S, Rodolfo M et al (2009) Mutational profiling of cancer candidate genes in glioblastoma, melanoma and pancreatic carcinoma reveals a snapshot of their genomic landscapes. Hum Mutat 30 E451-E459... 

Target Validation. Several approaches, including those that involve the use of arrays, can be taken to validate short-listed therapeutic candidates. Genes whose transcript profiles suggest additional analysis can be profiled using TMAs in tens to hundreds of tissues by immunohistochemistry [142]. TMAs can be used to identify heterogeneities between primary tumors and their metastases, as demonstrated in the analyses of erbB2 in primary and metastatic breast cancers... 

---