Candida tropicalis ATCC

Craft DL, KM Madduri, M Eshoo, CR Wilson (2003) Identification and characterization of the CYP52 family of Candida tropicalis ATCC 20336, important for the conversion of fatty acids and alkanes to a,(o-dicarboxylic acids. Appl Environ Microbiol 69 5983-5991. 

When (V-acetylprimaquine (175) was incubated for prolonged periods of time with Candida tropicalis (ATCC 20021) (232) and Streptomyces rimosus (ATCC 23955) (233), two unusual minor dimeric metabolites were obtained. These were identified as the methylene-linked compound 177 formed by C. tropicalis and the biaryl-linked compound 178 formed by 5. rimosus. Both dimeric metabolites were prepared synthetically to confirm their identities. 

All the fungi which were tested, i.e., Candida albicans (ATCC 140503 ), Candida tropicalis (ATCC 13803 ) and Candida krusei (ATCC 34135 ) successfully showed consistent ZOI in the presence of PANI- and PANI-doped with fluconazole. As the concentration of PANI- and PANI-doped with fluconazole increased, the susceptibility also increased for all fungi. The results elucidated the inhibitory concentration of PANI and PANI-doped with fluconazole on Candida tropicalis (ATCC 13803 ). There were no ZOI of PANI in dimethylsulfoxide which acts as a control. The ZOI were 7 mm for a concentration of 1.25 mg/ml, 8 mm for a concentration of 2.5 mg/ml, 9 mm for a concentration of 5.0 mg/ml and 11 mm for a concentration of 10 mg/ml. From this we can assume that the MIC of PANI for Candida tropicalis (ATCC 13803 ) is 1.25 mg/ml. The ZOI were 9 mm for a concentration of 1.25 mg/ml, 10 mm for a concentration of 2.5 mg/ml, 11 mm for a concentration of 5.0 mg/ml and 13 mm for concentration of 10 mg/ml. From this we can assume that the MIC of PANI-doped fluconazole for Candida tropicalis (ATCC 13803 ) is also 1.25 mg/ml [47]. 

FIGURE 18.4 Profiles of fed-batch fermentation of Candida tropicalis ATCC 13803 in a 3.5-L fermentor containing 1 L of YP medium (10 g/L yeast extract, 20 g/L bactopeptone), in which 105 g/L xylose and 32 g/L glucose were added initially. Fermentation environments were fixed at 30°C, pH 6, 500 rpm, and 1 vvm., dry cell mass , glucose A, xylose O, xylitol solid line, feeding of a concentrated xylose solution arrow, shift of dissolved oxygen content. 

FIGURE 18.5 (a) A diagram of cell-recycle operation system (Bae et al., 2004) and (b) a cell-recycle fermentation of Candida tropicalis ATCC 13803 in YP medium with initial concentration of 30 g/L glucose and 100 g/L xylose (Choi et al., 2000). The yeast cells were recycled with a hollow fiber membrane with 100,000 molecnlar weight cut-off. Feeding solution was composed of 750 g/L xylose, 200 g/L glucose, and 100 g/L yeast extract., dry cell mass , glucose A, xylose O, xylitol arrow, addition of feed solution. 

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