Candida arctica lipases

These excellent solvation properties were also used by Ganske et al. They were able to synthesize glucose fatty acid esters directly from glucose and fatty acid ester or free fatty acid, respectively. With their mixture of ionic liquid ([BMIM][Bp4] or [BMIM][PFs]) and tert-butanol the group found a system containing the sugar, the acid or acid ester, and the active enzyme Candida arctica lipase B at the same time [59,60]. 

Shen, Z., and Wijesundera, C. 2006. Evaluation of ethanolysis with immobilized Candida ant-arctica lipase for regiospecific analysis of triacylglycerols containing highly unsaturated fatty acids. J. Am. Oil Chem. Soc. 83 923-927. 

P. Saunders, J. Brask. Improved immobilization supports for Candida ant-arctica lipase B. In K. Loos (Ed.), Biocatalysis in Polymer Chemistry. Wiley-VCH, Weinheim, pp. 65-82. 2010. 

Shaken M, Engstrom K, Sandstrom AG, Backvall J-E. Highly enantioselective resolution of -amino esters by Candida ant-arctica lipase A immobilized in mesoceUular foam application to dynamic kinetic resolution. ChemCatChem 2010 2 534-538. 

The thermostability of the crude lipase produced by Candida ant-arctica is demonstrated in Figure 1. The remaining activities after incubation for 30 min at different temperatures in 50 irM Tris-maleate buffer, pH 7.0, were measured using olive oil as substrate at 40°C, followed by end-point titration of the liberated fatty acids. 

Salis A, Svensson I, Monduzzi M, SoUnas V, Adlercreutz P. 2003. The atypical lipase B from Candida ant-arctica is better adapted for organic media than the typical lipase from Thermomyces lanuginosa. Biochim Biophys Acta 1646 145-151. 

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