Calorimetric deconvolution models

Calorimetric Deconvolution Models and the Reversibility or Irreversibility of Overall Denaturation Processes. The deconvolution procedures used to analyze the thermograms presented in this study are based on equilibrium models, even though the overall denaturation process seen over a cycle of heating to a temperature above T and then cooling to below is, depending on the pH, either completely or partially irreversible. There is ample precedent in the literature for the application of equilibrium models in such cases, however. Convincing evidence has been presented... 

The above form of the set of equations indicates that, for various heat capacities there is no need to change the mathematical model of the calorimeter a change in C calls only for the introduction of new data in the deconvolution program. This can also be applied when the heat capacities of the calorimetric vessel content changes during the experiment (e.g. in the titration process). 

The same deconvolution was applied at all the peaks in the calorimetric trace, and as a result two thermograms are generated. The binding thermogram was used to calculate the binding parameters with the proper model (in this case, two equal and independent sites). Lastly, the kinetic constant for the chemical reaction at this temperature can be calculated from the response time, Ts as k=l/Ts,c. 

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