Butanoylation

The acetoacetyl group is then transformed to a butanoyl group by the reaction sequence illustrated m steps 2 to 4... 

The four carbon atoms of the butanoyl group originate m two molecules of acetyl coenzyme A Carbon dioxide assists the reaction but is not incorporated into the prod uct The same carbon dioxide that is used to convert one molecule of acetyl coenzyme A to malonyl coenzyme A is regenerated m the decarboxylation step that accompanies carbon-carbon bond formation... 

FIGURE 26 3 Mechanism of biosynthesis of a butanoyl group from acetyl and malonyl building blocks... 

Butylidyne Butyryl or butanoyl) Camphoroyl Carbamoyl Carbazolyl Carbazoyl Carbonimidoyl Carbonohydrazido preferred to carbohydazido or carbazido)... 

Butyryi chloride (butanoyl chloride) [141-75-3] M 106.6, f -89°, b 101-102°/atm, 1.026, Hp 1.412. Check IR to see if there is a significant peak at 3000-3500 cm (br) for OH. If OH is present then reflux with less than one mol equiv of SOCI2 for Ih and distil directly. The fraction boiling between 85-100° is then refractionated at atm pressure. Keep all apparatus free from moisture and store the product in sealed glass ampoules under N2. LACHRYMATORY - handle in a good fume hood. [Org Synth Coll Vol I 147 794/.]... 

Similar data were reported by Turro et al., [62,63] who synthesized a copolymer of AA with 1.5 mol% of 2-[4-(l-pyrene)butanoyl]aminopropenoic acid, 19 and studied the fluorescence quenching with Tl +, Cu2+, and 1 ions in aqueous solution. 

Acetyl-phenyl- 635 4-Amino-3,5-dimethyl-phenyl- 102 2-Amino-4-hydroxy-phenyl- 692 4-Amino-phenyl- 685 4-Benzylamino-phenyl- 102 2,4-Dinitro-phenyl- 692 4-(2-Hydroxy-benzylamino)-phenyl- 102 2-(2-Methyl-butanoyl)-phenyl- 635 4-Methoxy-2,6-dimethyl-phenyl-102 (Nitro-phenyl)- 635 2-Nitro-phenyl- 680, 692 4-Nitro-phenyl- 685... 

H 0 A-butanoyl-L-homoserine lactone, BHL or C4-HSL Aeromomas hydrophila Aeromonas salmonicida Pseudomonas aeruginosa, Serratia liquefaciens Extracellular protease, biofilm formation. Extracellular protease. Virulence factors - alkaline protease, cyanide, elastase, haemolysin, lectins, pyocyanin, rhaminolipid, RpoS Swarming, protease. 

---