Bugbead assays

The ability to combine small volumes with excellent sensitivity has made amperometric detection with RDE ideal for bead-based immunoassays. Direct comparison of the results obtained in the above Bugbead assay with those obtained with FIAEC detection showed that the detection limit of RDE was 2 orders of magnitude better than for EIAEC [53]. The other important feature of RDE is the extremely short detection time that could be reached by combining enzyme turnover with electrochemical detection. The RDE detection time of 2 min in the above Bugbead assay is over 10 times faster than the typical detection time for HAEC that requires a 20 min enzyme substrate incubation. It is also clear... 

We have carried out several successful sandwich immunoassays in this way, including assays for mouse IgG [50], ovalbumin [51], and an artificial microorganism called the Bugbead [52]. To illustrate the features of bead-based... 

The RDE current-time plots from the Bugbead immunoassay using the detection scheme described above are shown in Eigure 15. The curves correspond to assays of Bugbead standards ranging from 18 to 1.2 x 10 Bugbeads/ jL. The curves for the low concentrations are shown in larger scale in the inset. All the... 

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