Buffers Sorenson

The ionic species of the mobile phase will also affect the separation. This is shown in Table 4.3 by the difference in resolution values for magnesium chloride buffer compared to sodium sulfate buffer. In addition, calibration curves for proteins in potassium phosphate buffers are shallower than those generated in sodium phosphate buffers. The slope of the curve in Sorenson buffer (containing both Na and ) is midway between the slopes generated with either cation alone (1). Table 4.4 illustrates the impact of different buffer conditions on mass recovery for six sample proteins. In this case, the mass recovery of proteins (1,4) is higher with sodium or potassium phosphate buffers (pH 6.9) than with Tris-HCl buffers (pH 7.8). 

A quantity of the product is infiltrated into a glass fiber filter with the aid of a Buchner funnel and vacuum. If necessary, viscous and solid products can be diluted or comminuted in Sorenson s buffer, pH 7.0, to allow penetration into the glass fibers. Small squares of the filter (about 2 mm on a side) are cut and placed in PBS for 10 min. The squares are moved to a multiple well spot dish and covered with a 0.1%W/V aqueous solution of LBG, pH 7, for... 

Lysine Stock Dissolve 16.4 g of 1-Lysine in 300 mL distilled H20. Adjust pH to 7.4 with 0.1 M Na2HP04. Bring volume to 450 mL with distilled H20. Bring volume to 900 mL with 0.1 M Sorenson s Phosphate buffer. This may be stored at 4° C for up to 3 weeks. 

M Sorenson s phosphate buffer Stock Dissolve 7.176 g sodium phosphate monobasic monohydrate (NaH2P04-H20) in 100 mL distilled H20. Dissolve 49.4 g of sodium phosphate (NaP04) in 750 mL distilled H20. Mix the sodium phosphate monobasic monohydrate and sodium phosphate solutions in a 1 L cylinder. Bring volume to 1 L with distilled water. The pH should be 7.6. Do not adjust pH. This may be stored at room temp for up to 6 months. For use dilute with distilled water to 0.1 M. 

A differential pulse polarographic method for the determination of prednisolone in tablets is described. The method is more sensitive than dc polarography and the measurement of diffusion current is greatly simplified. Sorenson phosphate buffer pH 5.6 was used as the supporting... 

Properties Wh. to yish. wh. cryst. or amorphous powd., odorless readily sol. in Sorenson s sodium phosphate buffer sol n. sparingly sol. in water pract. insol. in alcohol, glycerin m.w. 23,000 pH... 

Giemsa stain 4% (v/v) Giemsa solution in Sorenson s buffer. This must be freshly prepared immediately prior to use. If a film appears on the surface remove it with filter paper. 

The optimum pH of the depolymerase reaction was determined by using buffers at increments of 0.5 pH units between pH 5.5 and 12.5. Sorenson s phosphate buffer was used between pH 5.5 and 7.5, tr is (hydroxymethyl) aminomethane-HCl buffer was used between pH 7.5 and 8.5, and Sorenson s glycine buffer was used above pH 8.5. The pH of the reaction mixtures was verified after mixing. 

Laccase from Polyporus versicolor (E.C. 1.10.3.2., 180 000 U/ml) was obtained from the Institute of Biochemistry, Academy of Sciences, Armenian SSR. Peroxidase (POD) from milk (E.C. 1.11.1.7., 340 pmol/l) was a ift from the Institute of Physiological Chemistry, University of Umea (Sweden). Other reagents used were of analytical grade. The background solution was a Sorenson phosphate buffer of pH 5.0 - 7.0 or 0.1 M acetate buffer of pH 5.5. 

---