Buffers coating carbonate/bicarbonate

Coating buffer 50 mM carbonate-bicarbonate pH 9.6, 0.02% sodium azide. 

ELISA coating buffer, carbonate/bicarbonate buffer, 0.02 M, pH 9.6. 

Figure 2. ELISA checkerboard titration of PCS coating antigen and PCS antibody. Conditions Varying concentrations of PCS antisera diluted in carbonate/bicarbonate buffer (pH 9.5), plates were washed with phosphate buffer pH 7.2, PCS antibody was diluted in PBS Tween 20, seconday antibody was IgG goat anti-rabbit with alkaline phosphatase label.

Coating Buffer The first step in an ELISA is immobilization of the analyte antianalyte antibody on the microtiter plate. Immobilization occurs passively through van der Waals forces and hydrophobic interactions between the protein and the plastic surface. Proteins have minimum net charge and maximum hydrophobicity near their isoelectric points (IEPs) thus, a coating buffer with a pH close to the IEP of the protein aids in its adsorption to the plate. Unfortunately, information about the IEP of the protein may not be available. In such situations, one should evaluate the suitability of commonly used coating buffers such as lOOmM bicarbonate carbonate buffer (pH 8 10), 50mM PBS (pH -7.4), or 50 mM acetate buffer (pH 5) [17,18],... 

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