Bubble injection leakage

Cell Loading. Sample and reference buffer are loaded into the cells using the same procedure described above for DSC to eliminate air bubbles. Erratic performance usually indicates either bubbles or leaking cells. The cells are overfilled outside the calorimeter to about 2 mm above the tip of the loading capillary and then inserted stepwise into the calorimeter to gradually equilibrate the cells and minimize perturbation of the calorimeter. The syringe is put into place at the end of the equilibration to minimize the effects of leakage from the tip of the injection needle. Total equilibration requires approximately 2 hr. 

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