Bromphenols

Bromphenol blue, tetrabromophenol-sulfonphthalein (indicator) dissolve 0.1 g in 7.45 mL 0.02 N NaOH and dilute with water to 250 mL or, 0.1 g in 100 mL 20% alcohol pH range yellow 3.6-4.6 violet-blue. 

SOLID-PHASE SPECTROPHOTOMETRIC AND TEST DETERMINATION OF CATIONIC SURFACTANTS ON PAPER FILTERS AS ION ASSOCIATE WITH BROMPHENOL BLUE... 

In such combining forms as bromo-, cyano-, chloro-, nitro-, the connective 0 is to be used invariably (with the exception of a few well-established words such as acetamide, cyanamide) as, Chlorbenzol, chlorobenzene Chlor-essigsdure, chloroacetic acid. This usage is by no means universal, but those who can not reconcile themselves to such spellings as bromoacetic should at least avoid the German forms bromphenol, acetphenetidine, etc., and use the connecting o before consonants,... 

Barr et al. Bromphenol blue 8 Cetyltrimethyl-ammonium bromide 267... 

Chen and Laidler [Canadian J. Chem., 37 (599), 1959] have studied the reaction of the quinoid form of bromphenol blue with hydroxide ions to give the carbinol form of the dye. The following values of the second-order rate constant at 25 °C were reported. 

Protein (total) Fast green pH2 Ninhydrin—Alloxan Schiff s Mercuric-bromphenol blue... 

The fading of bromphenol blue in the liquid phase is a second order reaction. The specific rate in liters/(mol)(sec) has been measured at 25 C as a function of pressure (Chem Laidler, Can J Chem 37 599, 1959) ... 

O ttflif If you were titrating a strong base with a weak acid, which of these indicators might be suitable bromphenol blue, bromthymol blue, or phenolphthalein (Refer to Figure 9.3.)... 

Suppose that you have the following indicators available bromphenol blue, methyl red, bromthymol blue, and phenolphthalein. Use Figure 9.3 to suggest an appropriate indicator for each of the following titrations. Do not do any calculations. 

Silica gel Hexane / ether / acetic acid (81/5/4) Bromphenol blue Benzoic acid. Sorbic acid. Salicylic acid 22... 

In the presence of 0.1 M potassium hydrogen phthalate buffer (adjusted to pH = 4.5), CHCI3,0.05 N NaOH for extraction, and 0.04% methanolic bromphenol blue for color production, chlorpromazine gives a blue color [31]. 

An ion-pairing spectrophotometric method has also been described [14]. This method involves the use of bromphenol blue, bromcresol purple, bromcresol green, bromthymol blue, or methyl orange, and extracting the ion-pair species into chloroform. The absorbance of the yellow product is determined at 420 nm. 

Bromophenol and Derivatives Bromophenol(called Bromphenol in Ger), Br.C6H4.OH mw 163.02. Three isomers exist and are described in the literature ... 

The experimental and predicted profiles for the adsorption rates of phenol and -bromophenol from an equimolar mixture of concentration 5 x 10-4 M are shown in Figure 12. The predicted profile for j>-bromophenol is in excellent agreement with the experimental data. However, for phenol there is some deviation after the Initial time period. The experimental adsorption rate for phenol appears to be faster than predicted for about 60 minutes after the first 15 minutes. Thereafter, the rate Is slightly slower than predicted. From an examination of the binary equilibrium data, this deviation may be attributed to the Inadequate correlation of the mixture equilibrium data in this region. The predicted and experimental total concentration profiles are shown In Figure 13. Initial concentrations of 2.5 x 10 4 M for phenol, and 5 x 10 4 M for j>-bromphenol were used in another rate study, the data from which are shown in Figures 13 and 14. The experimental and predicted curves are in fair agreement. 

P. (1987). Bromphenol blue nitrate reductase activity in Nicotiana plumbaginifolia. An immunological and genetic approach. Biochimie 69, 735-42. 

Loading solution (90% formamide, trace amounts of bromphenol blue and xylene cyanol)... 

The deprotected oligonucleotide synthetic product is precipitated twice in ethanol, and a 0.5 fig/fd solution in water is prepared (concentration is measured from a UV absorption spectrum). One microliter of the oligo-deoxynucleotide solution is mixed with 2 fd of 10X PL, 5 fd of [y-32P]ATP (or [y-35S]ATP), 1 fd of T4 polynucleotide kinase, and 11 fd water. After incubation at 37 ° (for 45 min with [y-32P]ATP or for 2 hr with [y-35S]ATP), the reaction is stopped by the addition of 150 [A of 5 M ammonium acetate, pH 5.5, and 130 fd water and 10 fd of the yeast tRNA solution are added to the mixture before precipitation with 1 ml ethanol. After chilling at —70° for at least 15 min, the precipitate is collected by centrifugation (12,000 g, 15 min), redissolved, and submitted to two additional cycles of precipitation-redissolution. Finally, the precipitate is redissolved in 20 fd of gel loading mix and the mixture analyzed on a 8% acrylamide-7 Af urea slab gel in IX electrophoresis buffer, until the bromphenol blue has reached the middle of the gel. 

---