Bromothymol Blue, Sodium Salt

Other Names Phenol, 4,4 -(3H-2,l-benzoxathiol-3-ylidene)fei5 [2-bromo-3-methyl-6-(l-methylethyl)-, S,S-dioxide, monosodium salt 3H-2,l-Benzoxathiole, phenol deriv. Bromthymol blue sodium CA Index Name Phenol, 4,4 -(l,l-dioxido-3H-2,l-benzoxathiol-3-ylidene)fcii[2-bromo-3-methyl-6-(l-methylethyl)-, monosodium salt CAS Registry Number 34722-90-2 Merck Index Number Not listed Chemical Structure 

Chemical/Dye Class Sulfonephthalein Molecular Formula C27H27Br205SNa Molecular Weight 646.38 pH Range 6.0-7.6 

Physical Form Orange-brown powder Solubility Soluble in water, ethanol UV-Visible (Xmax) 615 run, 392 run Melting Point 250°C Synthesis Synthetic method -  

Major Applications Lithium battery,3 textiles, antagonists  

Omdoiff, W. R. ComweU, T. K. Thymolsutfonephthalein, the intermediate acid, 4 -hydroxy-3 -isopropyl-6 -methyl-benzoylbenzene-2-sulfonic acid and some of their derivatives. J. Am. Chem. Soc. 1926,48, 981-993. 

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Bromothymol Blue sodium salt [34722-90-2] yellow-blue 0.05 (in water)... 

Bromothymol blue, sodium salt 6.0-7.6 70% ethanol Yellow Blue... 

Some textbooks state that the pH scale runs from 0 to 14, while others that it runs from 1 to 14. They both state that 7 is in the middle It seems that the pH scale can rvm from a practical value of around -1.6 to around 15.6. Typical wide-range indicator paper available in schools runs from 3 to 11, and the same is true for universal indicator solutions. The colour of pH 7 is green for some universal indicator solutions, but yellow for others. The recipe used for the mixture of indicators determines the actual colour seen. Yamada created and patented the first recipe (water, propan-l-ol, phenolphthalein sodium salt, sodium hydroxide, methyl red, bromothymol blue monosodium salt and thymol blue monosodium salt) in 1923. 

Fig. 4. — Monitoring of the Multiple Molecular Forms of Tomato Pectinesterase by Starch-gel Electrophoresis.98 [ENZ, detection of pectinesterase activity by paper print with pectin and Bromothymol Blue PROT, protein staining with nigrosin O, origin. Key A, 1 crude tomato extract after ammonium sulfate salting-out, and dialysis 2 pectinesterase fraction from column of DEAE-Sephadex A-50 3 and 4 pectinesterase fractions from column of Sephadex G-75. B, Two parts of the same gel after horizontal slicing 1, 500 fig of the isolated form of pectinesterase from a column of CM-Seph-adex C-50 with 175 mM phosphate-sodium chloride buffer 2, active fraction at 150 mM buffer 4 and 5, 250 fig and 1 mg of the isolated form of pectinesterase, respectively.]...

Alizarin Alizarin Red S Alizarin Yellow R Benzopurpurine 4B 4,4 -Bis(2-amino-l-naphthylazo)-2,2 -stilbenedisulfonic acid 4,4 -Bis(4-amino-l-naphthylazo)-2,2 -stilbenedisulfonic acid Brilliant Yellow Bromocresol Green Bromocresol Purple Bromophenol Blue Bromothymol Blue Chlorophenol Red Clayton Yellow Congo Red o-Cresolphthalein Cresol Red Crystal Violet Curcumin (Turmaric) p-(2,4-Dihydroxyphenylazo) benzenesulfonic acid, sodium salt... 

To 20 ml of chloroform in a separator add 5-0 ml of a solution of the sample containing not more than two micromols of univalent quaternary ammonium salt, 1 0 ml of bromothymol blue solution (an aqueous solution containing 0-15 per cent of bromothymol blue and 0 15 per cent of anhydrous sodium carbonate) and 20-0 ml of buffer solution, pH 7 5, prepared by diluting 7-5 ml of freshly prepared 0 1 M citric acid to 100 ml with 0-2M sodium phosphate (7-16 per cent Na2HP04,12H20). Shake vigorously for two minutes, allow the layers to separate, slowly invert the separator to mix the chloroform phase and allow to stand for... 

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