Bromopheneramine maleate

Related substance present in good number of official drugs may be determined by GLC method, for instance Bromopheneramine maleate Bronopol Cepahloridine Chlorocresol Chloroform Chloroxylenol., Cindamycin hydrochloride Griseofulvin Isometheptene mucate Levomenthol ... 

Materials Required Solution (1) dilute 1 volume of solution (3) to 200 volumes with a 0.005% w/v solution of N-phenylcarbazole (internal standard) in toluence 10 ml Solution (2) (add 5 ml of DW to 0.1 g of bromopheneramine maleate sample, make the resulting solution alkaline with 13.5 M ammonia, add 2.5 ml of toluene, shake for 5 minutes, centrifuge and use the upper layer) solution (3) (prepare it exactly in the same manner as solution- 2 but using the internal standard solution in place of toluene) Solution (4) (dissolve 10 mg of bromopheneramine maleate BPCRS in 5 ml of DW, make alkaline with 13.5 M ammonia, add 2.5 ml of toluene, shake for 5 minutes, centrifuge and dilute 1 volume of the upper layer to 20 volumes with toluene) ... 

Bronopol Same as under Bromopheneramine Maleate 1 pi Solution A Solution B Solutions (1), (2) and (3) Calculate ratio for sample and internal stand, from soln. (1) for soln. (3) ratio of area of any secondary peak and is not > y. 

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