Bromoacetamidophenyl derivatives

Bromoacetamidophenyl derivatives of thymidylic acid have been used to elucidate the structure of the substrate-binding site of Staphylococcus aureus nuclease. Similarly, bromoacetamidophenyl derivatives of the initiation codon, A-U-G, and the termination codon, U-G-A, have been employed as affinity probes of the ribosomal codon binding sites. The synthesis of these chemically reactive oligonucleotides combines chemical and enzymic methods as outlined for the synthesis of 5 -[4-(bromo-[2-i C] acetamido) phenylphospho ] adenylyl- (3 -5 ) -uridiylyl- (3 -6 ) guano-sine ( A-U-G) in Fig. 1. 

Bromoacetamidophenyl derivatives, 669 5 - [4-(Bromoacetamido) phenylphospho] -adenylyl-(3-5 )-uridiylyl-(3 -5 )-guanosine ( ATJG), 669, 670, 673... 

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