Bright state Document

T (H2O) = 2. 75 ms. The photophysical characteristics are weU documented (luminescence decay in D2O and at 77 K) residual water molecules ( H20 0.2-0.4) and triplet state energy of gadolinium complex (25,150 cm ) however the actual expected brightness upon laser excitation cannot be compared with other complexes since the absorbance at 337 nm is not reported. The conjugation of the complex with biomolecules was performed by its derivatization (as isothiocyanate) in relatively low yield using a tedious synthetic protocol. Unfortunately, for either the bis-pyrazolylpyridine or the bis-pyrazolylpyrimidine lanthanide complexes, no data are available concerning the stability in the presence of EDTA or competing ions (Mn Ca " ) or in potentially troublesome but basic buffer as phosphate buffer therefore, it is hard to evaluate the real usefulness of these complexes in a real bioassay, as in the HTS context. 

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