Bratton-Marshall reagent

Folic acid is detected by irradiating the chromatogram with broad-spectrum UV light for 30 min before reaction with Bratton-Marshall reagent (detection limit 200ng)[12]. 

The specific detection of aromatic nitro compounds is a second example. These can be converted by reduction to primary amines, which are then diazotized and coupled to yield azo dyes (cf. reagent sequence Titanium(III) chloride — Bratton-Marshall reagent ). Sodium nitrite —naphthol reagent, diazotized sulfanilic acid and other reagents specific for amino groups (e.g. ninhydrin, fluorescamine, DOOB, NBD chloride [9]) can also be used in the second stage of the reaction (Fig. 21). 

Primary aromatic amines are first diazotized by the action of sodium nitrite in acid solution and then coupled, for instance, with 1-naphthol to form azo dyes (cC Bratton-Marshall reagent, Vol. 1 a). ... 

Sodium nitrite + a-naphthol or Bratton-Marshall reagent... 

Reagents for detecting hydralazine were Dragendorff s reagent, alkaline dimethylaminobenzaldehyde, anisaldehyde, and Bratton-Marshall reagent. 

Methods based on HX (X = Br or I)-catalyzed denitrosation (g) HX denitrosation-TEA in water 5-80% Acetone in NOX (NO ) Griess or Bratton-Marshall reagent) Chemiluminescence to nitrosamides fully automated Applicable to most NOC 72... 

A colorimetric procedure for phenytoin developed by Dill et al24f provided the first reliable means for determining blood and tissue levels of this drug. The procedure involves quantitative nitration, reduction of the nitro group to a primary amine, diazotization and coupling with the Bratton-Marshall reagent. 

Polyolefin laminate Drug product stored in plastic hags, SPE preparation HPTLC. Plate = 10 x 20 cm silica gel. Mobile phase acetone - chloroform - concentrated sodium hydroxide (20 80 0.2). Photodensitometric detection, at 200 and 234 nm before derivitization, 388 nm after derivitization with ninhydrin and 580 nm after derivitization with Bratton/Marshall reagent. e-Caprolactam Eganox 1010 Butylhydrox)4oluene 4,4 -methylene dianiline 7... 

Detection Spraying with 6M HCl, heating for 10 min at 180°C, and spraying with Bratton-Marshall reagent [sodium nitrite and V-(l-naph-thyl)ethylenediamine dihydrochloride] purple-blue band 100 ng sensitivity. 

Kajganovic (90) have used Bratton-Marshall reagent for quantitation after separation and elution of sulfonamide mixtures containing sulfacetamide. The solvent system chloroform-methanol-25% ammonia solution (90 15 2.4) gives good separation. The results show a relative standard deviation of about 4%. Sarsunova et al. (86) have used chloroform-ethanol (100 8) as solvent system for the separation of sulfacetamide, sulfathiazole and sulfadimidine in tablets. The sulfonamides are assayed by extraction with acetone-ethanol, separation on alumina, and determination at 270 and 290 nm. The relative standard deviations of 2.5%, 4.1% and 3.2% have been obtained for the three components with six replicates. 

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