Branson cell disrupter

Sonication. Fermentor broths were reduced in volume via filtration to several liters or less. This reduced volume was then continuously circulated through a sonifer (Branson Cell Disrupter 185) ... 

For the specific amplification of C. moewusii genomic hydA DNA, a C. moewusii colony was scraped from an agar plate and resuspended in 10 mM Tris-HCl, 10 mM EDTA and 150 mM NaCl. After disrupting the cells (Cell disruptor B 15, Branson, Danbury, USA), 2 pi of the resulting suspension was used directly in the PCR. 

Protein was determined by the method of Lowry "et al," (1951) using bovine serum albumin as a standard. GSH was determined spectrophotome-trically as described by Hafeman "et al." (1974). Chlorophyll was determined as described by Mackinney (1941). The cells were disrupted in the cold with a sonifier Branson B-12 (60s, 70W) or by using a refrigerated Aminco French Press pressure cell and the 40000 x 20 min supernatant used as crude extract. 

---