Bovine cerebrosides

Sphingomyelin, bovine cerebrosides, psychosine and sphingo-sine were obtained from Supelco, Inc. Dihydrolactocerebroside and dihydroglucocerebroside were obtained from Miles Yeda Ltd. Psychosine and a new analog thereof were extracted from human brain tissue and separated by HPLC as their biphenyl carbonyl derivatives. 

Central nervous system myelin is enriched in certain lipids. Table 4-1 lists the composition of bovine, rat, and human myelin compared to bovine and human white matter, human gray matter, and rat whole brain [1] (see Ch. 3). While there are no absolutely myelin-specific lipids, cerebroside (galactosyl ceramide) is the most typical of myelin. With the exception of early development,... 

Figure 8. Field desorption mass spectrum obtained at 22 ma for a mixture of cerebrosides from bovine brain. Assignments of MH are discussed in the text and...

Phospholipid standards (Phospholipid kit) were purchased from Funakoshi (Tokyo, Japan). Cerebroside (bovine), D-a-phosphatidylcholine dipalmitoyl, and D-a-phosphatidylcholine distearoyl were purchased from... 

Calorimetry and Raman spectroscopy were used to measured the gel state of cerebrosides from bovine brain.The enthalpy of the gel-liquid crystalline phase transition for kerasin (15.8 kcal moF ) was markedly higher than that for phrenosin and unfractionated bovine brain cerebrosides ca. 7 kcal moF ). 

Figure 9.38 Methane chemical ionization spectra at 2.8Torr reactor 339 °C. (A) Dihydrosphingosine. (B) Ceramides (natural source). (C) Sphingomyelin (bovine). (D) Cerebrosides (bovine). Samples were applied to the belt continuously (Privett and Erdahl, 1978).

Fig. 150. Thin-layer chromatogram of phospholipids and other polar lipids of bovine milk [159 a]. 1 carbohydrate (lactose) and protein, 2 sphingomyelin, 3 phosphatidyl choline, 4 phosphatidyl serine, 5 phosphatidyl inositol, 6 phosphatidyl ethanolamine, 7 cerebroside dihexoside ( ), 8 cerebroside mono-hexoside ( ), P fatty acids, 10 neutral lipids. Adsorbent Silica gel HR. Solvents I, chloroform-methanol-water-28% aqu. ammonia (130 + 70+8 + 0.5) II, chloroform-acetone-methanol-acetic acid-water (50 + 20 + 10 + 10 + 5). Time 40 min in each direction. Indicator charring with chromic sulphuric acid solution...

Figure 5.16. Separation of hydroxy and non-hydroxy fatty acid methyl esters from cerebrosides of bovine brain on a fused silica WCOT column (25 m x 0.25 mm) coated with OV-101 [5]. The column temperature was 280°C and the flow-rate of the nitrogen carrier gas was 0.5 mL/min. (Reproduced by kind permission of the authors and of the Journal of Chromatography, and redrawn from the original paper).

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