Bovine aldose reductase

Bovine aldose reductase Inhibited by ahphatic, cychc and aromatic oximes, using benzyl alcohol as substrate 123... 

Pyrrolo[2,3-c]carbazole alkaloids (see Schemes 2.70-2.72) isolated from the dark green marine sponge, Dictyodendrilla sp., showed inhibitory activity against bovine lens aldose reductase catalyzing the reduction of aldoses to polyols which, on accumulation in cells, may result in diabetes. These alkaloids could provide prevention from such ailments (250). 

Several thieno[3,2-d]pyrimidine-2,4-diones 267 were evaluated in vitro for their ability to inhibit enzymatic activity on partially purified bovine lens aldose reductase. A high level of in vitro activity was demonstrated (ICjo 10"6 to 4 x 10 8 M) (91JMC1492 93MI1). 

Capillarisin Cirsimaritin Rhamno-citrin Artemisia Capillari Spica/Compositae Whole plant Three flavonoids and two coumarins, scoparone and scopoletin, were isolated from the ethyl acetate extract of A. capillari. These compounds have been tested for their inhibitory effect on bovine lens aldose reductase (bovine-LAR) and rabbit platelet aggregation. Increased aldose reductase activity and platelet aggregation are considered as two causes of diabetic complications. Capillarisin exhibited a potent inhibitory effect on bovine-LAR. Scoparone showed strong inhibitory effect on rabbit platelet aggregation [25],... 

Tissue Location and Role of Aldose Reductase in Animal Models of Diabetic Complications. Aldose reductase (AR) has been located immunohistochemically in many tissues of the dog and rat, most notably, in corneal epithelium, retina, optic nerve, kidney papillae, aortic endothelium and smooth muscle cells as well as peripheral nerve and lens. AR has also been measured in human and monkey retinal mural cells. These cells are thought to provide the structural support for retinal capillaries and their loss is the first abnormality seen in clinical diabetic retinopathy. In addition, AR-like activity has been reported in a human retinoblastoma cell line and sorbinil inhibits this activity in these cells. Finally, a recent report has demonstrated that AR is present in isolated capillaries from bovine retina and cerebral cortex. Therefore, AR appears to be present in all tissues which are uniquely susceptible to deterioration during prolonged exposure to the hyperglycemia of diabetes. Accumulation of the products of the polyol pathway, sorbitol and fructose, has been demonstrated in these tissues and, where tested, sorbinil and other AR inhibitors have been shown to inhibit this accumulation. 

Lens aldose reductase (bovine-LAR) inhibition Scoparone (27) and scopoletin (42) Isolated from Artemisia capillari and tested on bovine en me [221]... 

Okuda J, Miwa I, Inagaki K et al (1982) Inhibition of aldose reductases from rat and bovine lenses by flavonoids. Biochem Pharmacol 31 3807-3822... 

A series of 2-(5-((naphthalen-6-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl) acetic acid analogues have been prepared as aldose reductase inhibitors. In vitro inhibitory activities of bovine lens aldose reductase were determined by a conventional method (Scheme 9.7). 

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