Blood serum, soda

Glucose Luminol-H202-glucose-glucose oxidase pH 8.5 HTAB (reversed micelles) LDR 2.7 X 10-8-2.7 X 10-6 mol.L-1 Slope 1.58 RSD 4.27% LOD 2.7 X 10-8 mol.L-1 Human blood serum Soda... 

The determination of the enantiomers in blood serum was carried out in two stages. The first stage is to isolate and collect the racemic mixture from the serum. The second stage, was to resolve the collected racemate and to quantitatively determine the enantiomers present. Serum (1 ml) was added to 50 pi of a standard containing 200 ng of Imipramine and 400 pi of 5 N caustic soda. The mixture was shaken and 5 ml of -butyl ether added and the mixture shaken for a further 10 min. The mixture was then centrifuged for 10 min at 1200 and the aqueous layer frozen in a bath of solid CO and acetone. The ether layer was decanted from the container and evaporated to dryness at 45°C under a stream of filtered air. 

It follows, that a technique was required for the separation and estimation of both isomers present in blood serum and urine and a suitable procedure was developed by Susuki et al. [4]. The drug enantiomers were extracted in the following manner. A sample of serum or urine (1 ml) was treated with 100 pi of the standard solution (S,R-Vamicanide, containing 5 pg per ml for serum, 10 pg per ml for urine), 100 pi of distilled water and 1 ml of 0.1 M caustic soda. The mixture was then extracted with 5 ml of diethyl ether for 10 min with mechanical... 

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