Blocking Aldehyde Groups

Aldehyde groups are useful in facilitating modification or conjugation reactions, easily forming secondary amine linkages with amine-containing molecules in reductive am- 

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Generating Haworth formulas to show stereochemistry m furanose forms of higher aldoses is slightly more complicated and requires an additional operation Furanose forms of D ribose are frequently encountered building blocks m biologically important organic molecules They result from hemiacetal formation between the aldehyde group and the C 4 hydroxyl... 

Figure 1.124 Aldehyde groups may be blocked with Tris or ethanolamine using a reductive amination process.

The amino group on the glycine will bind to any free aldehyde groups left on the cells after fixation and prevent them from binding to the antibody or blocking solution, thus reducing the nonspecific background. 

For cases where the regioselectivity of lithiation cannot be biased in the required direction, silylation can be used to block acidic sites while further lithiations are carried out. The blocking silyl group is later readily removed with fluoride . This method was used to avoid a competing lateral lithiation in the synthesis of the aldehyde 665 (Scheme 251... 

Float the grid on 0.02M glycine m PBS for 3 min. This will block any free aldehyde groups in the tissue and prevent the antibodies from being fixed nonimmunologically on the tissue. 

Thus, the aldol route will be blocked, or partially blocked, if the only available aldehydes have fewer than two C-hydrogens. Hence, inclusion of "blocking" aldehydes of Group 1 or 2 in a browning reaction mixture should increase the proportion of free aldehyde produced and generally lower the chain length of aldol polymers formed. Overall, an increase in aroma volatiles should be observed, except with aldehydes of such low boiling point as to be able to escape physically and for which the aldol condensation acts as a restraint. 

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