Template bleeding

Gi bieeding Ticlopidine prolongs template bleeding time. Use with caution in patients who have lesions with a propensity to bleed (such as ulcers). Use drugs that might induce such lesions with caution in patients on ticlopidine. 

The active substance in bivalirudin (Hirulog), a direct thrombin inhibitor, is a 20-amino acids ynthetic peptide based on the hirudin template. In the Hirulog angioplasty study, 4098 patients with unstable or post infarction angina were randomized to bivalirudin or heparin before PTCA (14). The conclusion of this study was that there was no difference in the 30-day primary endpoint with either treatment. Patients randomized to Hirulog, however, did have a statistically significant reduced incidence of bleeding-related complications. 

Injury-Induced Arterial I.M.4.3 Template Bleeding Time... 

Template buccal mucosal bleeding time is carried out using the Simplate device. 

In addition, the template bleeding time is measured at various time intervals before and after drug treatment (depending on the route of administration) in the shaved inner ear using the Simplate device. Care is taken to select parts of the skin without larger vessels. 

TEMPLATE BLEEDING AN UNRESOLVED ISSUE IN MISPE PROTOCOLS... 

Other urgent improvements concern the mass transfer properties of the materials and bleeding of residual traces of template remaining entrapped in the material.4 The latter properties are influenced by the polymer morphology, which in turn is affected by the type and amount of cross-linking monomer, and the type of porogenic solvent. 

To avoid template bleeding, i.e., the bleeding of low levels of template from the MIP, thus precluding SPE applications in trace analysis.16 20... 

Unlike DNA diagnostics with clinical samples, where the amount of DNA in a volume of blood or other biological fluid can be estimated with little consequence, postpurification DNAquantification is of the utmost importance in forensic sample analysis. The target mass of DNA required for effective use of the commercially available STR kits is 1 ng. When a reduced mass of DNA is added to the PCR, stochastic effects that could include allelic dropout, may make interpretation difficult. Alternatively, an excess of DNA in the PCR (>5 ng) can result in off-scale peaks, pull-up (color bleeding usually due to off-scale peaks), increased levels of stutter (small peaks typically one repeat unit shorter than the STR allele, believed to be due to slippage of the primer or template during replication) or other artifacts in the subsequent electrophoretic separation. 

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