BLAST domain functionality analysis

Putative functional domains within the uncharacterized protein can be identified using the CDD search tools while performing protein-protein BLAST (BLASTP) alignments to obtain insight into possible functions of the predicted protein. The predicted protein sequence is used since amino-acid sequences are more evolutionarily conserved than nucleotide sequences, and therefore a broader search across all available sequence information from a variety of organisms can be conducted to identify potential functionality. Results from the BLASTP and CDD analysis (Fig. 10) have identified... 

First, the functions of all orphan gene products are predicted by BLAST or other protein analysis tools. NRPS and tailoring enzymes are differentiated. In addition, the substrates of the A domains can be predicted from their 10 letter codes 99,100 by bioinformatic tools such as NRPS Predictor101 although this is not a prerequisite for the in vitro reconstitution approach but a routine in NRPS characterization. 

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