Bisphosphoglycerate phosphatase

Bisphosphoglycerate is synthesized from 1,3-bisphosphoglycerate, a reaction catalyzed by 2.3-bisphosphoglycerate synthase. The enzyme 2,3-bisphosphoglycerate phosphatase catalyzes the hydrolysis of 2,3-bisphosphoglycerate to 3-phosphoglycerate. 

Ikura K, Sasaki R, Narita H, Sugimoto E, Chiba H. Mxiltifunctional enzyme, bisphosphoglyceromu-tase/2,3-bisphosphoglycerate phosphatase/phospho-glyceromutase, from human erythrocytes. Evidence for a common active site. Eur 1 Biochem 1976 66 515-22. 

Examination of the first partial reaction reveals that the mutase functions as a phosphatase—it converts 2,3-bisphosphoglycerate into 2-phosphoglycerate. However, the phosphoryl group remains linked to the enzyme. This phosphoryl group is then transferred to 3-phosphoglycerate to reform 2,3-bisphosphoglycerate. 

The mutase then functions as a phosphatase it converts 2,3-bisphosphoglycerate into 2-phosphoglycerate. The mutase retains the phosphoryl group to regenerate the modified histidine. 

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