Biotransformation enzymes, variants

Ulijn et al. identified an enzyme, capable of enantioselectively reducing the ketone, from their extensive collection of ADH variants further modification of the hit resulted in a biocatalyst that produces the desired (5)-alcohol in >99.9% ee at concentrations of 100 gL in a sofid-to-sofid biotransformation, where both starting material and product display only sparing solubility in the reaction medium. High conversions (>99%) are achieved by the substrate-coupled method, using 50 % v/v isopropyl alcohol concentrations to drive the reaction by continuous acetone removal (Scheme 1.55). The product can be easily isolated by filtration and washing. 

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