Biotin-sulfoxide, structure

The formal transfer of an oxygen atom is one way of describing the function of the Mo site in molybdoenzymes.5 The formation of dinuclear reduction products is a complication that causes difficulty in trying to model the mononuclear site.5,165 This difficulty can be overcome by the use of sterically demanding ligands that prevent the formation of the dinuclear complex,73 79,125 176 177 For example, the cycle shown in Scheme 3 can be effected without dimerization. Further, in this case DMSO and the enzyme substrate biotin sulfoxide, can serve as the oxo donor to form the Movl dioxo complex during the catalytic cycle.79,177 The Movl complex involved is discussed structurally above (Figure 7). 

Pierson, D. E., and Campbell, A., 1990, Cloning and nucleotide sequence of bisC, the structural gene for biotin sulfoxide reductase in Escherichia coli, J. Bacterial. 172 219492198. 

Figure 2 Structure of (-)biotin sulfoxide, (+)biotin sulfoxide, and biotin sulfone.

However, some of these structures show unusual features and a crowded active site. EXAFS studies of Rhodobacter capsulatus DM SO reductase show the expected four Mo-S ligands and one Mo-O bond arising from a serine residue plus one Mo=0 in the reduced Mo (IV) state of the active site. This oxo ligand is removed upon oxidation of the metal ion to Mo(VI). In the oxidized structme, an aquo ligand is postulated to coordinate the molybdenum ion (Fig. 11.17c) [126-128]. An equivalent restdt has been reported for BSO that catalyzes the reduction of D-biotin D-sulfoxide to D-biotin [129]. A similar overall catalytic mechanism is expected for nitrate reductases (Fig. 11.18), which catalyze the following reaction ... 

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