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Biotin-LC-hydrazide

An analog of this biotinylation reagent with a longer spacer arm also exists. Biotin-LC-hydrazide contains a 6-aminocaproic acid extension off its valeric acid group (Thermo Fisher). [Pg.526]

The increased length of this spacer (24.7 A) provides more efficient interaction potential with avidin or streptavidin probes, possibly increasing the sensitivity of assay systems. The reactions of biotin-LC-hydrazide are identical to those of biotin-hydrazide. [Pg.527]

Add biotin-hydrazide or biotin-LC-hydrazide to a final concentration of 5 mM. [Pg.527]

Add a sufficient amount of the hydrazide (e.g., 0.2 pg/ml of digoxigenin hydrazide (digoxigenin-succinyl-e-aminocaproic acid hydrazide) or 25pg/ml of Biotin-LC-Hydrazide ) and incubate for 1 h at RT. Wash three times for 10 min with Soln. D and block the membranes for 15 min with an appropriate blocking solution, e.g., 1% non-fat dry milk in Soln. D. Wash with Soln. D. [Pg.77]

See other pages where Biotin-LC-hydrazide is mentioned: [Pg.526]    [Pg.526]    [Pg.528]    [Pg.736]    [Pg.991]    [Pg.410]    [Pg.411]    [Pg.412]    [Pg.681]    [Pg.390]    [Pg.391]    [Pg.392]    [Pg.661]    [Pg.74]    [Pg.74]   


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Hydrazides biotin-hydrazide

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