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Biotin—Hydrazide Modification of Bisulfite-Activated Cytosine Groups

Biotin-Hydrazide Modification of Bisulfite-Activated Cytosine Groups [Pg.990]

Prepare 50 pg of a single-stranded DNA probe in 300 pi of 50mM sodium acetate, pH 4.5. [Pg.991]

Dissolve biotin-hydrazide in water at a concentration of lOmg/ml. [Pg.991]

Add sodium bisulfite to obtain a final concentration of 1M in the reaction medium. [Pg.991]

Remove excess reactants by dialysis against water at 4°C. [Pg.992]



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10- cytosin

Activating groups

Active groups

Biotin modification

Biotin-hydrazide cytosine modification

Biotin-hydrazide groups

Bisulfite

Bisulfite Activation of Cytosine

Cytosine

Group Activation

Group modification

Hydrazide modification

Hydrazides biotin-hydrazide

Modification biotin-hydrazide

Modification hydrazides

Of -biotin

Of hydrazide

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