Biotin diagnostic tests

Immunoassay has been widely applied in clinical diagnostics for many years. Diagnostic tests were mostly done using directly coated tubes, particles or microplates coated with antibodies or antigens via adsorption or chemical bonding. However, the test performance could adversely be influenced by the many parameters. Therefore, the avidin-biotin (AB) systems were introduced into the clinical laboratory to replace directly bound antibodies and antigens as solid phase matrices. 

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