Biosensors Using Intact Biological Receptors

Isolated nicotonic acetylcholine as well as plant receptors have been employed in receptrodes for the assay of acetylcholine and its antagonists, and auxin and toxin, respectively (Rechnitz, 1987 Thompson et al., 1986). 

Neutrophil leucocytes respond to various stimulants by the production of extensive amounts of superoxide anion, the so-called respiratory burst. A sensor for neutrophils based on the stimulation by IgG adsorbed on a pyrolytic graphite electrode has been described by Green et al. (1984). The liberation of superoxide anion as detected within 3 min at the sensor was related to the number of neutrophil leucocytes. The absence of an oxidation current in the presence of superoxide dismutase proved the formation of superoxide anion. 

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