Biopolymer Chromatography on Organic Monoliths

Due to their defined monomodal macropore distribution (see Section 1.2.1), monolithic stationary phases, based on polymerization of organic precursors, are predestined for efficient and swift separation of macromolecules, like proteins, peptides, or nucleic acids, as their open-pore structure of account for enhanced mass transfer due to convection rather than diffusion. In fact, most of the applications of organic monolith introduced and investigated in literature are directed to analysis of biomolecule chromatography [29]. 

Summary of Commercially Available Monolithic HPLC Columns together with Their Preferred Field of Application 

Support Material Chemical Nature Brand Name Company Dimension Preferred Field of Application 

Monolithic silica Cj3 reversed phase Chromolith RP-18 Merck KgaA 100 X 4.6 mm 50 X 4.6 mm 25x4.6 mm Swift RP analysis of small molecules 

Monolithic silica Cj reversed phase Chromolith RP-8 Merck KgaA 100x4.6 mm Analysis of small molecules and hydrophobic peptides 

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