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Protein biophysical characterization

Fig. 14A-C. Guanosine-nucleotides for biophysical characterizations of Ras proteins A gua-nosinediphosphate with a methylanthraniloylester (in grey) at the 3 -position of the ribose B guanosine-5 -[/3,y-imido]-triphosphate (GppNHp) C guanosine-5 -0-(3-thiotriphosphate)... Fig. 14A-C. Guanosine-nucleotides for biophysical characterizations of Ras proteins A gua-nosinediphosphate with a methylanthraniloylester (in grey) at the 3 -position of the ribose B guanosine-5 -[/3,y-imido]-triphosphate (GppNHp) C guanosine-5 -0-(3-thiotriphosphate)...
Jung Y-S, Gao-Sheridan HS, Christiansen J, et al. 1999. Purification and biophysical characterization of a new [2Ee-2S] ferredoxin from Azotobactervinelandii, a pnta-tive [Fe-S] cluster assembly/repair protein. J Biol Chem 274 32402-10. [Pg.64]

Biophysical characterization showed that a single HRP II protein bound 17 molecules of heme [35]. In an in vitro heme polymerization assay, HRP II promoted the synthesis of hemozoin, while controls, such as the proteins bovine serum albumin and lysozyme or the homopeptides polyhistidine, polylysine, and polyasparagine, did not. FT-IR analysis of the reaction product showed the characteristic vibrations of hemozoin. The polymerization activity had a pH maximum near 4.0, which dropped off precipitously near the pKa of histidine. The heme polymerization... [Pg.334]

Kreiter R, Kleij AW, Klein Gebbink RJM, van Koten G (2001) Dendritic Catalysts. 217 163-199 Kuhlmann J, Herrmann C (2000) Biophysical Characterization of the Ras Protein. 211 61-116... [Pg.239]


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See also in sourсe #XX -- [ Pg.122 ]




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Biophysical characterization

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