Bioorganic and biocatalytic reactions

Masaya Miyazaki, Maria Portia Briones-Nagata, Takeshi Honda, and Hiroshi Yamaguchi 

Microreactors in Organic Chemistry and Catalysis, Second Edition. Edited by Thomas Wirth. 

Moreover, studies that report on biomolecular synthesis in microfluidic reactors are also covered in this chapter. 

Another application of microfluidic enzymatic reactors is in the enormous and diverse challenges of proteomic investigations. Enzymatic microreactors present proteomics with a valuable analytical tool for protein analysis. Most of applications of IMERs are currently directed at protein analysis by protein digestion and peptide mapping. 

An important analytical application of enzymatic microreactors is their use in biocatalysis, in order to transform a difficult-to-measure analyte into an easily measurable form. This could be represented by microreactors designed for digestion of proteins to convert them to more readily measured peptides. Applications of microreactors in continuous-flow chemistry have expanded rapidly over the past two decades, with numerous reports of higher conversions and yields compared to conventional batch equipment. In this chapter, a comprehensive discussion on the most recent trends in the development of enzymatic microreactors and their current applications are covered. 

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In the following discussion, applications of enzymatic microreactors will be presented. Comprehensive and up-to-date references are included that will give readers access to detailed information on the arising trends in the development of enzymatic microreactors for bioorganic and biocatalytic reactions and their current applications for applied analytical chemistry and biochemical studies. 

This chapter presents a wide coverage of the state-of-the-art applications of microfluidic reactors for bioorganic and biocatalytic reactions. The development of microreactors for biomolecular syntheses although much slower compared to (iTAS is expected to continue to improve. Most biomolecules are expensive, and therefore high reaction yield and eflEdency are desired for their synthesis. The main challenge that remains for microreactor biomolecular syntheses is the integration of various components for chemical reactions such as synthesis, analysis, extraction, separation, and concentration. 

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