Biomolecule-functionalized nanoparticles interaction with

Au NPs functionalized with biomolecules can be synthesized using different methods, depending on factors inducing the interactions promoted between the nanoparticle and the biomolecule. These interactions can be classified as electrostatic adsorption, chemisorption and covalent binding and, finally, specific affinity interactions. Some examples are given in the following paragraphs (Scheme 3.23). 

The third group of Au NP-biomolecule interactions are referred to as specific affinity interactions and include nanoparticles functionalized with groups that provide affinity sites for the binding of bio molecules such as proteins and oligonucleotides. 

The bottom-up techniques described herein are based on the use of nanosize building blocks to fabricate precisely organized solids at various scales. The final architecture of the solid, and the way these blocks combine with each other, can be conveniently adjusted by the synthesis conditions, the selection and modification of these nanoblocks, and their chemical functionality. The spontaneous arrangement of individual nanoblocks is generally obtained via self-assembly through weak interactions. The control over the organization of these components allows for the incorporation of nanoparticles, biomolecules, or chemical functionalities inside the solid structure in highly precise locations. 

Functionalization of Nanoparticles with Biomolecules by Specific Affinity Interactions... 

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