BRET, Bioluminescent resonance energy transfer

Pfleger, K. D. and Eidne, K. A. (2006). Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat. Methods 3, 165-74. 

Xu, X., Soutto, M., Xie, Q., Servick, S., Subramanian, C., von Arnim, A. G. and Johnson, C. H. (2007). Imaging protein interactions with bioluminescence resonance energy transfer (BRET) in plant and mammalian cells and tissues. Proc. Natl. Acad. Sci. USA 104, 10264-9. 

Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc. Natl. Acad. Sci. USA 97, 3684-3689. 

Angers, S., Salahpour, A., Joly, E., Hilairet, S., Chelsky, D., Dennis, M. and Bouvier, M. (2000). Detection of b2 -adrenergic receptors dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proceedings of the National Academy of Sciences, USA, 97, 3684-3689. 

Like FRET, bioluminescence resonance energy transfer (BRET) is based on non-radiative energy transfer between a donor and an acceptor. However, in BRET, the donor is a luminescent molecule, excited by the enzyme Renilla Luciferase (Rluc), and not a fluorescent molecule. The BRET acceptor can be a fluorescent protein like green fluorescent protein (GFP) or YFR If the enzyme and the fluorescent protein are in close proximity (d < 10 nm), an energy transfer will occur between the Rluc substrate (coelanterazine) and the fluorescent protein, leading to emission from the later. 

There are two main types of RET, bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET). In the former, the donor molecule is luminescent (27,28)... 

Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions. Nat. Protoc. 1, 337-345. 

The bioluminescent resonance energy transfer (BRET) technique is based on the energy transfer between a luciferase enzyme acting as donor and a fluorescent protein acting as acceptor (Pfleger Eidne, 2006). The classical... 

Bioluminescence Bioluminescence resonance energy transfer BRET Microfluidics Thrombin... 

Bioluminescence resonance energy transfer (BRET) is a form of Eorster resonance energy transfer (RET). It is the non-radiative transfer of energy from an excited state donor to a ground state acceptor. The efficiency of energy transfer is highly dependent on the distance between the donor and acceptor moieties and their relative orientation with respect to each other. The photon emissions from the donor and receptor molecules during BRET reaction can be measured to quantify assay products. 

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