Bioluminescent bioreporter

Nivens, D.E. McKnight, T.E. Moser, S.A. Osbourn, S.J. Simpson, M.F. Sayler, G.S., Bioluminescent bioreporter integrated circuits Potentially small, rugged and inexpensive whole-cell biosensors for remote environmental monitoring J. Appl. Microbiol. 2004, 96, 33-46. 

DEVELOPMENT AND TESTING OF BACTERIOPHAGE-BASED BIOLUMINESCENT BIOREPORTERS FOR THE MONITORING OF MICROBIAL PATHOGENS IN THE SPACECRAFT ENVIRONMENT... 

G. Phage-amplified bioluminescent bioreporters for the detection of food-borne pathogens. Proceedings of SPIE 2004. 

BIOLUMINESCENT BIOREPORTER INTEGRATED CIRCUIT SENSING OF THE CHEMICAL AND BIOLOGICAL SPACECRAFT ENVIRONMENT... 

Figure 1. A bioluminescent bioreporter integrated circuit (BBIC)...

Simpson ML, Sayler GS, Patterson G, Nivens DE, Bolton E, Rochelle J, Amott C, Applegate B, Ripp S, Guillom MA. An integrated CMOS microluminometer for low-level luminescence sensing in the bioluminescent bioreporter integrated circuit. Sens Actuators B 2001 72 135-41. 

Ripp S, Daumer KA, McKnight T, Levine LH, Garland JL, Simpson ML, Sayler GS. Bioluminescent bioreporter integrated circuit sensing of microbial volatile organic compounds. J Ind Microbiol Biotechnol 2003 30 636-42. 

Ozen A, Montgomery K, Jegier P, Patterson S, Daumer K, Ripp S, Garland J, Sayler G. Development of bacteriophage-based bioluminescent bioreporters for monitoring of microbial pathogens. Proc SPIE 2004 5270 58-68. 

Figure 2. A) Genetic organization of the pKAl catabolic plasmid. Genes of the upper naphthalene regulatory system encode for proteins that mediate the conversion of naphthalene to salicylate. Salicylate is then further degraded to TCA cycle intermediates. B) In Pseudomonas fluorescens HK44, genes within the lower pathway were replaced with genes of the lux cassette to produce a bioluminescent bioreporter sensitive to naphthalene and salicylate.

Table 1. Chemical and physical agents capable of detection by luxCDABE bioluminescent bioreporters ...

Figure 4, Minimum detectable concentrations of toluene and naphthalene as a function of integration time for the prototype BBIC containing either the Pseudomonas putida TVA8 (toluene) or Pseudomonas fluorescens 5RL (naphthalene) bioluminescent bioreporters.

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