Biological Sample Preparation and Modes of Data Collection

Biological Sample Preparation and Modes of Data Collection 

Sample preparation is perhaps the most critical part of a successful IR microspectroscopy experiment In the same way that the IR microscope can be used in a number of ways to collect spectra, so too can sample preparation can approached in a variety of ways. As biological materials are most frequently probed in either transmission mode or reflection mode, these methods will be described here. Other methods are also available, however, such as grazing incidence and attenuated total reflection (ATR) [1]. 

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