Biological evaluation, selectins

Kretzschmar, G, Toepfer, A, Hiils, C, Krause, M, Pitfalls in the synthesis and biological evaluation of sialyl Lewis mimetics as potential selectin antagonists. Tetrahedron, 53, 2485-2494, 1997. 

Kaila N, Janz K, DeBemardo S, Bedard P, Camphausen R, Tam S, Tsao D, Keith J, Nickerson-Nutter C, ShUling A, Young-Sciame R, Wan Q (2007) Synthesis and biological evaluation of quinoline saMcyUc acids as P-selectin antagonists. J Med Chem 50 21-39... 

Many biological processes take place under dynamic rather than static conditions. For example, the ability of the selectins to mediate the dynamic process of leukocyte rolling is a critical aspect of their function yet most selectin inhibitors have been evaluated in static assays. A smdy evaluating multivalent L-selectin inhibitors revealed dramatic differences in their activities in static cell free versus dynamic cell rolling assays. 

---