Biohydrogen anaerobic bacteria

Dark fermentation is the fermentative conversion of organic substrate to biohydrogen, it is a complex process manifested by a diverse group of bacteria by a series of biochemical reactions involving three steps similar to anaerobic conversion. Dark fermentation differs from photofermentation because it proceeds without the presence of light. 

Cis-9, trans-ll CLA is produced directly as the first intermediate in the pathway of biohydrogenation of linoleic by the action of linoleic add isomerase, an enzyme of the anaerobic rumen bacteria Butyrivibrio fibrisolvens (15). This enzyme has an absolute substrate requiranent for a cis-9, cis-12 diene structure and a free carboxyl group, and exhibits maximum activity with the substrates linoleic and linolenic acids (16). Ruminants cannot produce PUFA and the amount of... 

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