Biogenesis of photosystem II

Five of the eight subunits of photosystem II reaction center are synthesized on chloroplast ribosomes. The three Tris-soluble subunits are synthesized on cytoplasmic ribosomes as larger precursors and are transported into the chloroplast by a vectorial processing mechanism [29, 119-121,133]. The genes coding for all the 

Most of the membrane protein complexes are quite stable and exhibit relatively slow turnover of synthesis and decomposition. Due to the harsh reaction catalysed 

2 Nelson, N. and Riezman, H. (1984) Biogenesis of Energy-Transducing System.s. New Comprehensive Biochemistry (Ernster. L.. ed.) Vol. 9, pp. 351-376, Elsevier. Amsterdam. 

3 Hauska. G., Hurt, E., Gabellini, N. and Lockau, W. (1983) Biochim. Biophys. Acta 726, 97-133. 

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BIOGENESIS OF PHOTOSYSTEM II IN C3 AND C4 PLANTS - A MODEL SYSTEM TO STUDY DEVELOPMENTALLY REGULATED AND CELL-SPECIFIC EXPRESSION OF PLASTID GENES... 

Biogenesis of photosystem II during greening of etiolated spinach seedlings is a stepwise process and controlled post-transcrlptionally... 

Work on the biogenesis of photosystem II in spinach was done, while P.W. was in the laboratory of Dr. R. Herrmann (University of Munich). The financial support of the Deutsche Forschungsgemeinschaft (SFBs 184 and 189) and Fonds der Chemischen Industrie is gratefully acknowledged. We thankDr. K. SteinmuUer for carefully reviewing the manuscript. 

Biogenesis of Photosystem II in C3 and C4 Plants - A Model System to Study Developmen-tally Regulated and Cell-Specific Expression of Plastid Genes 483... 

The Biogenesis and Assembly of the Major Antenna Complex of Photosystem II (LHCIIb) in Barley 661... 

Zhang L, Paakkarinen V, van Wijk KJ et al. Biogenesis of the chloroplast-encoded Dl protein Regulation of translation elongation, insertion, and assembly into photosystem II. Plant Cell 2000 12 1769-1781. 

Thus, photosystem II biogenesis in greening spinach seedlings is a stepwise process. The extrinsic polypeptides of the water-splitting complex accumulate without being attached to a functional reaction center. Moreover, even the reaction center components cytochrome b-559, D1 and D2 are assembled in a non-coordinate manner. 

Photosystem (PS) II in the procaryotic cyanobacteria is structurally similar to that in higher plants and contains around 20 different polypeptide subunits [1]. The reaction center of PSII, harboring the key components for the charge separation, consists of the D1 and D2 polypeptides [2,3]. Our studies concern the biogenesis of PSII in the cyanobacterium Synechocystis 6803. We are particularly interested in the role of the D1 polypeptide in the assembly process. We have previously described [4] the construction of a S. 6803 mutant where synthesis of the Dl polypeptide has been inactivated by insertional mutagenesis of the psbA gene family, encoding the Dl polypeptide. A preliminary functional characterization of that mutant showed that the PSII activity was severely perturbed while PSI activity was unaffected [4,5]. The structural analysis revealed that despite the lack of the Dl and D2 polypeptides, mutant thylakoids still retained several intrinsic PSII polypeptides [1]. 

THE BIOGENESIS AND ASSEMLY OF THE MAJOR ANTENNA COMPLEX PHOTOSYSTEM II (LHCIIb) IN BARLEY. 

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