Biodegradation dichloromethane

Leisinger, T., Bader, R., Hermann, R., Schmid-Appert, M. Vuilleumier, S. (1994). Microbes, enzymes, and genes involved in dichloromethane utilization. Biodegradation, 5, 237-48. 

Oxidation of cyclohexane-1,4-dione with MCPBA in dichloromethane (DCM) affords oxepane-2,5-dione, which the authors claim as a precursor of a novel class of versatile semicrystalline biodegradable (co)polyesters <2002MM7857, 2003MM2609>. 

Another approach to extend the action of proteins is to develop biodegradable polymeric microspheres. Owing to their excellent biocompatibility, the biodegradable polyesters, poly (lactic acid) (PEA) and poly(lactic-co-glycolic acid) (PEG A), are the most frequently used biomaterials to achieve sustained action [204, 205]. Polymeric microspheres are commonly prepared by the solvent extraction/evapora-tion methods [206]. In brief, protein in a solid or liquid form is mixed with a polymer (dissolved in an organic solvent, e.g., dichloromethane) to prepare a solid-... 

Microspheres of the biodegradable, triblock copol3rmer (PLGA-PEG-PLGA, Mw = 4000, 1500-1000-1500 by NMR) were prepared in two methods microsphere A (Msp A aqueous-based) and microsphere B (Msp B dichloromethane). For both microspheres, an equal amoxmt of Zn-insiilin was loaded ( 4% of pol3nner mass). In vitro release studies were carried out with both. As shown in Fig. 6, Msp A exhibited a continuous and nearly complete release of insiilin over 3 weeks. The first phase of insulin release (the first 10 days) from Msp A seems to be dependent more upon diffusion, indicated by the slight decrease in the release rate over time. Then, after day 10, the insulin release rate turned to an... 

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