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Biochemists Use the Auxotrophs Isolated by Geneticists

Finding intermediates in a pathway is only half the problem. The biochemist must also isolate and characterize the enzymes of the pathway. To this end, extracts are made from either normal or mutant cells. These extracts are tested in conjunction with different intermediates to see whether the extracts can carry out the conversion of one intermediate [Pg.489]

Immediate consequences of a point mutation in a biosynthetic pathway. When the mutation leads to an inactive enzyme, the chain of reactions leading to the end product in the pathway is broken, and frequently large amounts of intermediate are produced, accumulate in the cell, and may leak to the environment. [Pg.489]

Sometimes the investigation of a pathway is complicated by the fact that common intermediates are used in the synthesis of more than one amino acid. In such cases intermediates may be converted into more than one product by different enzymes, each product serving as an intermediate for a particular amino acid. Mutants containing defects in enzymes required for the synthesis of more than one amino acid usually require the addition of two or more amino acids to reestablish cell growth. [Pg.489]


See other pages where Biochemists Use the Auxotrophs Isolated by Geneticists is mentioned: [Pg.487]    [Pg.489]   


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