Bioaffinity specificity

Immunoaffinity chromatography is probably the most highly specific of all forms of bioaffinity chromatography. However this technique has some disadvantages such as this technique relatively high cost, leakage of ligands may accur from the column and sometimes the desorption procedure results in partial denaturation of the bound protein [24]. 

Complementary structures of biological materials, especially those of proteins, often result in specific recognitions and various types of biological affinity. These include many pairs of substances, such as enzyme-inhibitor, enzyme-substrate (analog), enzyme-coenzyme, hormone-receptor, and antigen-antibody, as summarized in Table 11.2. Thus, bioaffinity represents a useful approach to separating specific biological materials. 

Metal chelate and bioaffinity chromatography based on the affinity of the protein for a specific molecule that can be incorporated in the stationary phase (not commonly used for food analysis)... 

Membranes used for separation are thin selective barriers. They may be selective on the basis of size and shape, chemical properties, or electrical charge of the materials to be separated. As discussed in previous sections, membranes that are microporous control separation predominantly by size discrimination, charge interaction, or a combination of both, while nonporous membranes rely on preferential sorption and molecular diffusion of individual species. This permeation selectivity may, in turn, originate from chemical similarity, specific complexation, and/or ionic interaction between the permeants and the membrane material, or specific recognition mechanisms such as bioaffinity. 

Antigen specific recognition Bioaffinity Association of immobilized antigen on binding site... 

Figure 13.11. Bioaffinity electrospray FTICR mass spectrometry. The isolation and mass spectrometricidentificationof receptor-specific ligands are carried out entirely in the mass spectrometer without chromatography or other separation steps.

CSPs Based on Target-specific Bioaffinity Systems... 

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