Binding of Bile Acids to Anion-Exchange Resins

Binding of Bile Acids to Anion-Exchange Resins 

There is evidence (18, 19) that the fraction of resin sites entering into mass-action equilibrium with the bile salt anions may not be constant but increases gradually as the concentration of the bile salt anions in the resin increases. The increase in binding could also indicate some form of a cooperative process. A possible explanation is that sodium cholate and sodium 

TABLE III. Recovery of 24-C Mabeled Bile Acids from Amberlyst XN-1006 (20)  

Bile acid Amount added (cpm) Amount recovered (cpm) Percentage recovery 

Acids added at pH 11 to a 1 x 5 cm column of resin in the OH form. After washing with water and aqueous alcohol the bile acids were eluted with 150 ml of 0.2 M ammonium carbonate in 80% ethanol. The specific activity of the cholic, cheno-deoxycholic and deoxycholic acids was approximately 10, 8, and 3 /(c/mg, respectively. 

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C. Binding of Bile Acids to Anion-Exchange Resins... 

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