Binding continued

Wright (1953) has shown that the acid-binding capacity of horn is almost identical with that of wool, but insufficient data are available, particularly with respect to amide content, to permit this value to be related to the amino acid composition of hom (Table XIV). Harrold and Pethica (1958) have observed that normal callus absorbs a little more than 1 meq of dodecyl sodium sulfate per gram in the absence of salt at pH 6.6 and almost 3 meq per gram at a total ionic strength of 0.2. Binding continues to a maximum at a concentration above the critical micelle point of dodecyl sodium sulfate. There appears to be no relationship between the large absorption in the presence of salt and the amino acid composition of callus (Table XVI). 

Specificity The specificity of the antiserum was determined by measuring the cross reactivity with substances having a structure similar to that of theophylline. The cross reactivity is stated as the ratio of the theophylline concentration to the concentration of the cross-reacting substance at 50% inhibition of the maximal binding, (continued)... 

The nature of opioid binding continues to be explored. 3-48 jj, brain and spinal cord, u 6 sites predominate, and k sites represent only 10 of total binding. Both 6 and u receptors mediate antinociception. The antinociceptive profiles of p and k agonists were... 

In these studies, we packed cotton into empty columns and eluted the elastase over the cotton derivatives with buffers of physiological strength. Much of the elastase did not bind to the cotton sorbent, but that which 41 was not easily desorbed. Elastase did not desorb even when 1.0 M of NaCl was used as an eluent. Residual elastase was desorbed using 6 M urea (18). As more elastase was passed over the sorbent, less and less binding occurred, but binding continued to take place as long as... 

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