Buffer Bicine

BICINE is a compound containing a tertiary amino group whose relevant pAT is 8.3 (Figure 2.18). Given 1 L of 0.05 M BICINE with its tertiary amino group in the uiiprotoiiated form, how much O.l N HCl must be added to have a BICINE buffer solution of pH 7.5 What is the molarity of BICINE in the final buffer ... 

Figure 7.17 Hydrolysis of oleic anhydride catalyzed by spontaneously formed oleic acid vesicles at 40 °C, (a) during the first 3 h, and (b) during a long observation time. A vesicle suspension (10 ml in 0.2 M bicine buffer (pH 8.5)) was overlaid with 0.25 mmol oleic anhydride and 0.025 mmol oleic acid. The increase of the concentration of oleic acid/oleate is plotted as a function of reaction time. Initial concentration of oleic acid/oleate 0 mM ( ), 5 mM ( ), 10 mM (o), 20 mM ( ). For an initial oleic acid/oleate concentration of 20 mM, the concentration of oleic anhydride (A) present in the vesicles during the reaction is also plotted (b, right axis). (From Walde et al, 1994b.)...

Figure 10.15 Intensity-weighted size distribution of POPC in 0.2 M bicine buffer solution, pH 8.5 0.5 ml solution of 1 mM 30 nm extruded POPC vesicles mixed with 0.5 ml solution of 1 mM 200 nm extruded POPC vesicles. The measuring angle is 90°. (From Cheng and Luisi, 2003.)...

Figure 10.16 Effect of Ca + on the turbidity change upon mixing oleate vesicle solutions 0.25 ml 1 mM 60 nm radius extruded oleic acid vesicles + 0.25 ml 1 mM 200 nm radius extruded oleic acid vesicles + 1.5 ml bicine buffer. Calcium ion concentration (a) 0 mM (b) 1 irtM (c) 2.5 mM (d) 5 mM (e) added excess EDTA to (d). (Adapted from Cheng and Luisi, 2003.)...

Figure 10.19 Effect of pre-added vesicles on the formation of oleic acid/oleate vesicles. Turbidity measured at 500 nm (1 cm path length) is plotted as a function of time, T=2°C. (a) 62 p,l of 80 mM aqueous sodium oleate was added to 2.438 ml of0.2Mbicine buffer, pH 8.8 ([oleic acid/oleate] = 2 mM). (b)62 p,l of 80mM aqueous sodium oleate was added to 2.438 ml of a 2 mM oleic acid/oleate 100 nm vesicle suspension (0.2 M bicine buffer, pH 8.8 ([oleic acid/oleate] = 4 mM). (c) Turbidity of 2 mM oleic acid/oleate 100 nm vesicles , (d) the same as (b), but using a 50 nm vesicle suspension, (e) Turbidity of 2 mM oleic acid/oleate 50 nm vesicles . (Modified from Blochiger etal, 1998.)...

Dilute the test sera 100-fold with 50 mM Bicine buffer, pH 8.2. (Samples with very high or very low antigen concentrations are diluted accordingly.)... 

Figure 3. Freeze-fracture electron microscopy analysis of the vesicle size distribution in the case of the spontaneous vesiculation of oleic acid/oleate. (A) Vesicles formed from the hydrolysis of 25 mM oleic anhydride (overall concentration) at 30 °C, yielding 50 mM oleic acid/oleate. (B) Vesicles extruded throughout 50 nm diameter filters. (C) Vesicles formed upon hydrolyzing 20 mM oleic anhydride (same conditions as in A) in the presence of pre-added extruded vesicles B—all in 0.2 M bicine buffer pH 8.5. For details see ref. 8.

Assay of activity An aliqot of the crude extract was preincubated in 50 mM bicine buffer pH 8 with 20 mM MgCU 5 20 mM NaHCOT... 

RuBP carboxylase present in crude extracts was activated with 20 mM MgCl2 and 10 mM NaHCO and 10 mM DTT in 50 mM Bicine buffer pH 8 for 15 min. The extracts were further treated with 10 mM R-5-P and/or 2 mM ATP and/or 1 mM RuBP as mentioned in Table for further 30 min. Aliquots were withdrawn and tested for RuBP dependent activity. 

Figure 15.1 Release of compound 8 from 7 catalyzed by TK/BSA. Controls 7 (lOOmM), TK extract (1 unit ml" ) A, 7 in bicine buffer pH 8.2 +, 7 in -(N-morpholino) propanesulfonic acid (MOPS) buffer pH 7.2.

As mentioned above, it has been found earlier [14] that extensive dilution of 80mM oleic acid-oleate suspensions prepared in 0.2 M bicine buffer (pH 8.5) increased the... 

Figure 19.7 Dilution-induced change in the size distribution of oleic acid-oleate vesicles at pH 8.5 (0.2 M bicine buffer) (a) nonextruded vesicles (b) vesicles extruded through polycarbonate membranes with a mean pore diameter of 0.2 pm.

Figure 9. Fluorescence decay of the donor, MNA, in MNA-BPTI in 50% glycerol in 0.05M Bicine buffer, pH 7.5 at -30°C. The experimental curve was fitted to a monoexponential decay function. The shortest pulse,L(t), is the trace of the excitation pulse. The second pulse, F(t), shows the experimental and calculated donor fluorescence decay curves- The lower inset shows the deviation between the two curves, and the upper right inset shows the autocorrelation of the residuals-These show that the decay rate is raonoexponential (RMS of fit 0-0030)-...

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