Benzylamine oxidase

In benzylamine oxidase there is evidence that the amine undergoes transamination with the pyridoxal prosthetic group to give a pyridoxamine, which is then oxidized by dioxygen to give H202 and NH3. The role for the copper is one of activation of the substrate.1346... 

Barker, R., Boden, N., Cayley, G., Charlton S. C., Henson, R., Holmes, M. C., Lelly, I. D., and Knowles, P. F., 1979, Properties of cupric ions in benzylamine oxidase from pig plasma as studied by magnetic resonance and kinetic methods, Biochem. J. 177 2899302. 

Buffoni, F., and Blaschko, H., 1964, Benzylamine oxidase and histaminase purification and crystallization of an enzyme from pig plasma, Proc. Roy. Soc. B, 161 153. 

Buffoni, F., Blaschko, H., "Benzylamine Oxidase and Histamine Purifica-... 

Table 1. Alignment of amino acid sequences of several copper amine oxidase around the position of topa quinone. The sequences were obtained by translation the corresponding cDNAs except for the enzymes from porcine kidney and porcine serum and the benzylamine oxidase from Hansenula polymorpha where they were determined by automated Edman degradation of peptides. Homologous consensus sequence around the cofactor is underlined, the tyrosyl precursor of topa quinone is shown as y.

Copper-depleted bovine serum amine oxidase has been recently reconstituted with Co(II) ions. The benzylamine oxidase activity of the enzyme was increased to 20% on incorporation of cobalt. Furthermore, Co(II) restored to nearly native level the intensity of the absorption spectrum and the reactions with phenylhydrazine or benzylhydrazine, which had been slowed down or abolished, respectively, in Cu(II)-depleted samples. The amine oxidase activity of the Co(II)-derivative, which cannot form a semiquinone radical as an intermediate of the... 

Benzoic acid, mercapto-lithographic printing plates, 101 Benzonitrile hydrolysis metal catalysis, 449 Benzoquinone dioximates metal complexes non-integral oxidation states, 144 Benzylamine oxidase, 700 Benzyl halides carbonylation... 

Type 2 Cu2+ and Cu2+ in superoxide dismutase, benzylamine oxidase, and galactose oxidase are fallacious. 

S-mono-oxygenase and the reduced enzyme requires the presence of additional reductant for hydroxylase activity. The deaminating enzyme benzylamine oxidase consists of two subunits and has two tightly bound, but well separated,... 

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