Benzophenone-4-iodoacetamide

Benzophenone-4-iodoacetamide is water-insoluble and should be pre-dissolved in DMF or another organic solvent prior to adding an aliquot to an aqueous reaction mixture. Stock solutions may be prepared and stored successfully if protected from light. 

Benzophenone-4-iodoacetamide has been used to study the 100-KDa U5 snRNP protein (hPrp28p) and its interactions (Ismaili et al., 2001). 

Benzophenone-4-maleimide is a heterobifunctional photoreactive cross-linker that has sulfhydryl reactivity similar to benzophenone-4-iodoacetamide discussed in the 

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Figure 5.33 Benzophenone-4-iodoacetamide reacts with sulfhydryl-containing compounds to give thioether linkages. Subsequent photoactivation of the benzophenone residue gives a highly reactive triplet-state ketone intermediate. The energized electron can insert in active C—H or N—H bonds to give covalent crosslinks.

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