Radiolabeled benzamides

The preparation of alkyl, alkoxy, and radiolabelled benzamides of the current invention is provided (1). 

The covalent binding property of benzamides has been exploited in the development of cell-based competitive binding assays that measure the ability of other antitubulin agents to inhibit binding of radiolabeled benzamides to j -tubulin in whole cells. The tritiated S-enantiomer of zoxamide has been used to study the zoxamide binding site in the Oomycete Phytophthora capsid [7]. Tritiated analogs 2 and 3 (RH-4032 and RH-5854, Fig. 16.1.2) have been used in similar assays in plant [4] and mammalian cells [6], respectively. 

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